A White House official said Biden's comments did not reflect a policy shift.
Speaking alongside Japanese Prime Minister Fumio Kishida, Biden said any effort by China to use force against Taiwan would "just not be appropriate", adding that it "will dislocate the entire region and be another action similar to what happened in Ukraine."
China has stepped up its military provocations against democratic Taiwan in recent years aimed at intimidating it into accepting Beijing's demands to unify with the communist mainland.
"They're already flirting with danger right now by flying so close and all the manoeuvres that are undertaken," Biden said of China.
Under the "one China" policy, the US recognises Beijing as the government of China and doesn't have diplomatic relations with Taiwan. However, the US maintains unofficial contacts including a de facto embassy in Taipei, the capital, and supplies military equipment for the island's defence.
Biden said it is his "expectation" that China would not try to seize Taiwan by force, but he said that assessment "depends upon just how strong the world makes clear that that kind of action is going to result in long-term disapprobation by the rest of the community."
He added that deterring China from attacking Taiwan was one reason why it's important that Russian President Vladimir Putin "pay a dear price for his barbarism in Ukraine," lest China and other nations get the idea that such action is acceptable.
Fearing escalation with nuclear-armed Russia, Biden quickly ruled out putting US forces into direct conflict with Russia, but he has shipped billions of dollars in US military assistance that has helped Ukraine put up a stiffer-than-expected resistance to Russia's onslaught.
Biden's comments came just before he formally launched a long-anticipated Indo-Pacific trade pact that excludes Taiwan.
White House national security adviser Jake Sullivan confirmed on Sunday that Taiwan isn't among the governments signed up for the Indo-Pacific Economic Framework, which is meant to allow the US to work more closely with key Asian economies on issues like supply chains, digital trade, clean energy and anti-corruption initiatives. The inclusion of Taiwan would have irked China.
Sullivan said the US wants to deepen its economic partnership with Taiwan on a one-to-one basis.