Project scientist Hal Weaver, from the Applied Physics Laboratory at Johns Hopkins University in Maryland, US, said: "New Horizons is on a journey to a new class of planets we've never seen, in a place we've never been before.
"For decades we thought Pluto was this odd little body on the planetary outskirts; now we know it's really a gateway to an entire region of new worlds in the Kuiper Belt, and New Horizons is going to provide the first close-up look at them."
When New Horizons was brought out of hibernation 260 million kilometers from Pluto it was described as a "watershed event".
Scientists received a signal telling them the probe's pre-programmed computer had switched it to "active" mode that took four hours and 26 minutes to reach the Earth.
Since its launch on January 19, 2006, New Horizons has spent 1873 days - about two-thirds of its flight time - in hibernation to save wear and tear on electrical components and reduce the risk of system failures.