The Libyan capital was braced for fresh violence after a day in which at least 37 were killed and more than 400 wounded in a confrontation outside a militia headquarters.
In some of the bloodiest fighting since the overthrow of Muammar Gaddafi, a Misrata militia unit opened fire on protesters who had massed outside their Tripoli base, demanding they leave the city.
Hospitals were overwhelmed with the dead and wounded and Prime Minister Ali Zeidan appealed for calm, declaring three days of national mourning.
The United Nations and European Union evacuated non-essential staff from Libya yesterday, with foreign embassies going into security lockdown.
Eyewitnesses said that anti-aircraft weapons were fired at the protesters outside the base in the city's Gharghur district, amid conflicting reports of who fired first. The demonstrators, including women and teenagers, many carrying white placards and flags, fled the firing seeking the shelter of nearby houses.