She believes there should have been a referendum to define the meaning of marriage in the constitution, rather than the voluntary postal survey that returned a 61.6 per cent "yes" vote.
Hanson used the 1967 referendum over recognising indigenous Australians as an example of a change which had created unintended consequences such as "reverse racism".
"We have now made laws that give Aboriginals more rights than other Australians," Hanson said.
"They are treated totally different under the law.
"People did not vote for that, hence my concerns about this plebiscite," she said.
Hanson also took aim at the "disgraceful" Safe Schools programme, which she said was "messing around with the minds of young children".
NSW One Nation senator Brian Burston said he would have voted "no" in the survey, but never received a form.
"I was one of those Australians robbed and denied the opportunity of expressing my democratic right," Burston said.