In comparison, the bomb detonated over Hiroshima in 1945 released about 15 kilotons of energy.
The new analysis by 38 North, run by the US-Korea Institute at the Johns Hopkins School of Advanced International Studies, found North Korea's test may have been much stronger.
Updated seismic data showed the magnitude of the resulting earthquake was greater than initial estimates - between 6.1 and 6.3. That means the yield of the latest test was roughly 250 kilotons, reported 38 North's Frank V. Pabian, Joseph S. Bermudez Jr. and Jack Liu.
In other words, the North Korean test may have been almost 17 times stronger than the bomb detonated over Hiroshima. This is close to what 38 North previously calculated as the maximum yield that could be contained at the underground Punggye-ri test site.
This new estimate by 38 North is much higher than initial estimates from US intelligence sources and allies. The United States intelligence assessment put the blast at 140 kilotons.
Experts at the Center for Nonproliferation Studies in Monterey, California, said that the size of the earthquake triggered by the explosion also suggested that the bomb could have had a force in the hundreds of kilotons.
The Pentagon declined to comment, calling it an intelligence matter.
A US intelligence official said the 38 North analysis is consistent with the range of estimates by the intelligence community. The Air Force Technical Applications Center's early estimate was a range of 70 to 280 kilotons, based on the possible magnitude, the official said.
Satellite imagery showed the test resulted in many more landslides than after any of the previous five tests, according to the 38 North analysis.
North Korea described the device it had detonated as a hydrogen bomb designed to be carried by a long-range missile capable of reaching the US mainland. The international community widely condemned the test and within 10 days, the UN Security Council unanimously approved its toughest sanctions on the country to date.
In the wake of the North Korean test, both the United States and South Korea are highlighting their own military readiness.
Defense Secretary Jim Mattis was traveling Wednesday to Minot Air Force Base in North Dakota, the centre of the American nuclear arsenal, with more than 100 land-based nuclear missiles and aircraft.
Meanwhile, the South Korean Air Force yesterday conducted its first live-fire drill to test its preemptive strike capability, according to the South Korean Defense Ministry.