Snubbed, he then placed his hand on Melania's backside and attempted to steer her down the stairs.
The awkward encounter was the second in as many days, after video emerged Monday of when the first couple touched down in Tel Aviv.
A short clip showed Trump clearly reach out to his wife, who was walking a step behind him, and try to grab her hand.
But Melania slapped her hubbie's hand away without even breaking stride or showing any sort of emotion.
Trump then continued to walk ahead with Prime Minister Netanyahu and his wife, Sara.
It was unclear if this unexpected resistance was the result of any personal turmoil.
with his wife as they left Tel Aviv on Monday night.
Video showed the first lady as she wrapped two of her fingers around one of her husband's fingers for a few seconds while leading him towards their awaiting helicopter.
She also got involved in a four-way handshake with the Netanyahus as they posed for a press photo.