Riot police with shields and batons herded them into the corner of a raised terrace and beat them, reports on Italian television said.
Many dropped 4m into a courtyard to escape the blows.
Angry residents of Lampedusa surrounded the town hall, calling on the Mayor, Bernardino De Rubeis, to take a stronger line against the refugees. The local population of 5000 has often been outnumbered by migrants.
The mayor responded by producing a baseball bat from a drawer in his office and saying, "I have to defend myself and am ready to use it".
"It's like a war zone," he added, calling on the Government "immediately to send helicopters and ships to evacuate the Tunisians".
The Italian Government has made it clear that it believes most of the Tunisians are economic migrants and wants forcibly to repatriate them.
The Corriere della Sera newspaper quoted the Interior Minister, Roberto Maroni, as saying: "Within the next 48 hours all the illegal immigrants on Lampedusa will be transferred from the island and then repatriated."
The United Nations refugee agency warned last week that the situation on Lampedusa was deteriorating because of the prolonged detention of Tunisians and people fleeing Libya, without any moves to determine whether they might qualify for asylum.
Yesterday, the UN urged Italy to speed up the transfer of refugees from Lampedusa to more permanent centres. It said they were harming themselves and staging protests.
Until the recent mass influx of north Africans, Lampedusa was a quiet community that relied on fishing and summer tourism for a living.
The endless flow of arrivals and the catastrophic effect this has had on tourism have caused tempers to fray.
- Independent