Police say they are investigating to find out whether the delegates knowingly took the banned drug, or were the victims of a macabre prank.
When an ambulance was called to the conference last Friday, paramedics found delegates staggering around, talking incoherently and suffering from hallucinations.
Others were curled up on the ground with severe cramps. The paramedics were so concerned they called for support, and a staggering 160 emergency workers converged on the site, including helicopter teams.
"There must have been a multiple overdose," Torsten Passie, a member of a German government commission on narcotics, told NDR television.
"That argues against the people being aware what they were taking."
The drug "heightens the emotional experience", Mr Passie said. "An overdose can cause delusions and psychosis-like symptoms."
Those affected included doctors, as well as homeopaths and alternative medicine practitioners, according to reports.
The owner of the seminar centre distanced her team from the incident.
"We didn't have anything to do with it," Stekfa Weiland said.
The leading German alternative medicine association disowned the conference.
"I dissociate myself quite strongly from what happened at Handeloh," Heinz Kropmanns, president of the Association of German Naturopaths, told NDR.
"If I find out one of our members has participated, he will be thrown out of the association."
Police said any possible prosecution would depend on the result of investigations into whether the delegates had knowingly taken the drug.