She told 3AW his wife was discharged from hospital last week, and the man was discharged on Tuesday night.
The joy was twofold for the hospital, with the team responsible for treating the country's first coronavirus case also in charge of caring for Victoria's last, the Herald Sun reported.
Dr Stuart told the newspaper there were "mixed emotions" as doctors farewelled the man, explaining battling the virus had been a "rollercoaster ride".
"The whole treating team have gone on that journey with them, from being really concerned to now waiting out for us to release them as the last person from isolation in Victoria," she said. "It's a pretty remarkable story."
Deputy chief health officer Allen Cheng said the achievement was certainly a milestone for Victoria.
"It doesn't mean the threat is gone, but certainly a milestone considering we had 7880 active cases on August 11," he tweeted.
Premier Daniel Andrews also announced on Tuesday there were no new cases recorded overnight.
The last positive cases of coronavirus in Victoria were on October 30, when four infections were recorded.
Victoria has not recorded more than 25 consecutive days of zero new COVID-19 cases since before the first case was diagnosed on January 25.
The state has recorded 20,345 coronavirus cases and 819 deaths since the start of the pandemic, with 19,526 people having recovered as of Tuesday.
Mr Andrews said it was great news for the final patient and their family.
"It presents us with an opportunity to again thank all of those nurses and doctors, hospital cleaners, ward clerks and the whole health team for the amazing work that they've done throughout this global pandemic event," he said on Tuesday.
"Even with this run of zero days, no active cases, very strong testing performance and people who are just doing the right thing and playing their part, until the vaccine arrives, this is something that's going to be with us for the foreseeable future."
A total of 9960 COVID-19 tests results were received in the past 24 hours, an increase from the 7261 on Monday.