At least two Ukrainian women died in the crash, Ukraine’s Foreign Ministry said in a statement.
“An official of the Consulate of Ukraine in (the Czech city of) Brno is at the scene and in constant contact with rescue and law enforcement agencies,” it said.
Among the people who died were two Slovak women, the Slovak Foreign Ministry said.
The drivers of both trains survived, the local CTK news agency said.
Transport Minister Martin Kupka said the main track between Prague and the eastern part of the country had to be closed while authorities investigated.
It was only partially reopened nine hours later and the state-run train company, Czech Railways, advised that passengers should avoid using the route for the whole day.
The corridor in Pardubice, where the trains collided, is vital for Czech Railways.
Martin Drapal, a spokesperson for a state agency that investigates train crashes, said the driver of the train carrying passengers failed to halt the train at a stop sign.
He said it was not immediately clear if that was caused by human error or a technical problem.
Prime Minister Petr Fiala called the crash a big tragedy and offered his condolences to the families of those killed.
So did Radim Jančura, the owner of RegioJet, who said his company was ready to compensate the passengers.
- AP