Mould agreed to take a look at it and after weeks of painstaking cleaning and the removal of a top coat of 18th century paint, the work was verified by Dr Christopher Brown, one of the world's leading authorities on Anthony van Dyck.
MacLeod, who helps to run a retreat house in the Peak District, said: "It's been an emotional experience and it's such great news. It's wonderful that new church bells hopefully will be pealing out to commemorate the centenary of the [end of the] First World War in 2018."
Bruce said: "It's everyone's dream to spot a hidden masterpiece, I'm thrilled that my hunch paid off. To discover a genuine van Dyck is incredibly exciting. I'm so pleased for [MacLeod]."
The Flemish artist became the leading court painter in England under Charles I and his works hang in Buckingham Palace, Windsor Castle and in museums and galleries around the world.
A self-portrait recently sold for £12.5 million, prompting a fundraising campaign to try to save the painting for the nation.
The work discovered on the show is a portrait of a Brussels magistrate. It is believed to have been painted as part of the artist's preparation for a 1634 work showing seven magistrates, which was eventually destroyed in a French attack on the city in 1695.