"It confirms the passionate response of our discerning clientele to this new and unique product niche, combining luxurious self-contained villas on a private estate with complete services on tap."
Hollywood watchers might be interested to know Harrison Ford and Calista Flockhart spent time there early this year.
Demi Moore and Ashton Kutcher reportedly stayed last year, and the 75ha estate was the setting for part of season 13 of US reality TV show The Bachelor.
Ms Biskind travelled to Thailand to receive the award, describing it as "one of the most exciting nights in my career".
"It was truly a humbling experience. I felt so proud of everyone who has contributed and those who are working in the creation of our dream that is Eagles Nest," she said.
Prices for one of five luxury Eagles Nest villas range from $2346 a night to $20,439 a night during peak season.
For that, you'll receive a bottle of champagne, a fruit platter, a minibar, and a gourmet breakfast.
A high-speed internet connection is also thrown in.
You can also employ the services of a personal chef, personal trainer, massage therapist or chauffeur if you're willing to shell out a little extra.
World Luxury Hotel Awards executive manager Marinique Truter said it was one of the highest accolades a hotel could receive, and one that says "more about the property than any brochure, picture or personal testimonial could express".