As the deadline for the ban loomed, there had been a sharp spike in the number of people arriving to hike up the monolith, with a number of photos showing lengthy lines up the trail going viral.
According to the BBC, only 16 per cent of visitors to Uluru actually climbed it in 2017, when the upcoming ban was first announced, but that number has surged as the deadline drew nearer.
In fact, the ABC reports there were an extra 10,000 visitors to Uluru-Kata Tjuta National Park per month in the six months leading up to the climb's closure.
Yesterday, a clip posted on Twitter by ABC journalist Oliver Gordon showed crowds waiting at the base of the site at 7am.
The video revealed the queue snaking from the carpark all the way to the beginning of the climb, with commenters likening the crowd to a "conga line".
It attracted thousands of views and comments, with some describing the final mad rush to conquer Uluru as "disgusting", "selfish" and "disrespectful" to the traditional owners.
"You'd have to be really, really keen to insult and offend Traditional Owners to even consider climbing in such crowded conditions," one Twitter user posted, while another wrote: "Can any one of these climbers give me a good and valid reason why they feel they must do this? Why should you not be utterly ashamed and embarrassed? Anyone?"
The climb was established in 1964 on the steep western face of the rock, and from October 28, the chain handhold that guided tourists up the rock for decades will be dismantled.
From tomorrow onwards, heavy fines of up to $10,000 will be introduced for anyone who ignores the new law.
Over the decades, dozens of people have died while climbing Uluru for a range of reasons, including falls, dehydration and health issues.
In 2018, a Japanese tourist died while trying to climb one of the steepest parts of the rock, and last month, One Nation leader Senator Pauline Hanson got stuck while climbing Uluru in protest against the ban.
Earlier this month, a young South Australian girl fell at least 20 metres while descending from the summit after visiting the site with her parents and younger brother.
The 12-year-old lost her footing and fell on the lower section of the climb, near where the chain is located.
This month, Central Land Council chief executive Joe Martin-Jard told Sky News tourists had been increasingly using Uluru as a toilet.
He said that was one of the reasons behind the decision to outlaw the climb, along with the site's cultural significance and safety concerns.
"They've wanted to see it closed for a very long time, for spiritual reasons, for cultural reasons, but if you speak to them they'll also tell you that it's for safety reasons, they've had to take down bodies off the rock, people have fallen off the rock and it really hurts them when they see visitors being hurt," Mr Martin-Jard told Sky News.
"They're a bit disappointed with people going to the toilet once they're up there and leaving things like children's kimbies (nappies) behind, and when we have the rare event of rain that pee and crap flows down the rock into very fragile water holes and rock holes that animals drink from."