The death of about 20 brown trout in Mill Creek, near Arrowtown, is being investigated by the Otago Regional Council.
Chief executive Peter Bodeker said the council's environmental officers had collected water samples at several points along the creek, near Speargrass Flat, last week and again yesterday, both up and downstream of where the dead fish were found.
A report was received by Fish & Game on June 2, and the ORC notified. More dead fish were found during a site visit on June 7.
The water samples were being analysed by two laboratories to establish the presence of pesticides, or other chemicals, and heavy metals, as well as the overall quality of the water in the creek and an adjacent wetland.
Results from that analysis were expected in a week, while results of autopsies on two of the dead fish - sent to the Cawthron Institute in Nelson- were expected in a fortnight.