The latest surveillance test came back clear after the affected fisheries and other areas that had not been surveyed before were checked.
"Continued surveillance will ensure the ministry can move quickly if a new infection appears in the future," says Mr Gwyn.
Foveaux Strait fisheries had their surveillance frequencies increased from three to six months by the MPI.
Bonamia ostreae can be fatal for flat oysters. It has been in New Zealand since at least 2015, when it was detected in the Marlborough Sounds and Nelson.
MPI and Stewart Island farmers completed the removal of oyster lines from Big Glory Bay on 6 September. Removal operations in Marlborough are continuing.