A beef and sheep farm near Masterton in the Wairarapa has tested positive for the cattle disease Mycoplasma bovis.
The confirmation takes to 36 the total number of infected properties nationwide, said the Ministry for Primary Industries.
The farm was located by tracing animal movements from other M.bovis-affected farms, MPI said in a statement.
All cattle on the property will be killed in agreement with the farmer around timing, MPI said.
The government and farming sectors have agreed that all cattle on infected properties should be culled as part of a drive to eradicate the disease, first diagnosed in the South Island last July.