About 20 brown trout found dead in Mill Creek, near Arrowtown, were electrocuted because of a faulty submersible pump, the Otago Regional Council says.
Earlier this month, ORC chief executive Peter Bodeker said the council was investigating the cause of the fish deaths after being first notified by Fish and Game on June 2.
More were found during a site visit on June 7.
The council's environmental officers collected water samples at several points along the creek, near Speargrass Flat, on two occasions, both up and downstream of where the dead fish were found.
Those samples were analysed by two laboratories to determine the level of pesticides, other chemicals and heavy metals, as well as the overall quality of the water in the creek and a neighbouring wetland.
The analysis found no high levels of chemicals or heavy metals, except aluminium in the vicinity of the pump.