"This is extremely disappointing and unacceptable behaviour, and we are exploring what our enforcement options are.
"These beautiful birds are unique to New Zealand, mainly breeding on South Island braided rivers. With current trends they would further decline of around 70 per cent over the next 30 years."
Davey said in recent months there have been about six separate incidents of motorbikes or four-wheeled drive vehicles driving into the birds' nests.
"Two motorbikes came racing through a group of young gulls and later I found a number of dead birds near that area. We can't prove it was the motorbikes but I think it was."
The group has motion-detecting trail cameras set-up at the river.
"Another incident recently we caught on camera was a four-wheeled drive which was driving straight up to the gulls and they all took off in a big cloud and the vehicle just kept going straight through it.
"There are still a number of young birds that can't fly yet."
Davey said the rivercare group and Environment Canterbury have been working to solve the problem for years.
They monitor the area closely, and there is signage installed along the river advising park users about the nesting area, and concrete blocks are installed at entry points to deter vehicles, Hughey said.
"The nesting area has been cordoned off since September to protect the colony, and will be open in two weeks when the birds have fledged.
The birds' nesting season usually runs from about July-February each year, Davey said.
Under the Wildlife Act, penalties for hunting, killing or disturbing absolutely protected wildlife can be up to five years imprisonment or a $300,000 fine.
"It is just reckless and absolutely unacceptable behaviour."
• If you see anyone disturbing the colonies contact ECan on 0800 324 636.