One thousand cattle on a newly-identified infected Waimate property will be culled, on top of 4000 cattle already scheduled for destruction because of Mycoplasma bovis.
The latest farm to test positive is one of two in the Waimate district placed under restrictions last week.
Testing was ongoing on the second property, and a third farm in the area was placed under the same controls yesterday, while further testing was done to determine if it was infected.
All three farms were in the same district as the Van Leeuwen Dairy Group properties, the Ministry for Primary Industries' response incident controller David Yard said.
The culling of about 4000 dairy cattle from five Van Leeuwen Dairy Group properties had been signalled after an outbreak of the bacterial disease, which can cause mastitis, abortion, pneumonia and arthritis.