A second similar-sized venture, the $15.5million Lindis River development, was announced last month.
Hortinvest project and orchard development manager Ross Kirk said the third project at Mt Pisa cemented a long-term vision for the industry.
"New Zealand has distinct key competitive advantages such as the climate and conditions, proximity to markets and free trade agreements with Asia."
A total of 53,000 trees will be planted in the Mt Pisa project over the next three years and planting is set to begin this winter.
The orchard is expected to reach full mature production by the 2025-2026 summer.
Planting for the Lindis River project is also set to begin this winter.
Kirk said because of their elevation, at 300m above sea level, readings from weather stations at Lindis River and Mt Pisa sites indicated they fared better than other areas during harsh October frosts last year.
Hortinvest hoped to deal with any possible staff shortage issues by long-term plans to develop "an accommodation and labour hire model" that would support its projects.
Otago Chamber of Commerce chief executive Dougal McGowan said the scale of the project could provide greater certainty for workers.
"With large projects it gives you the opportunity to not only employ people short term and then distribute them to other areas after the cropping and pruning has been done, but it also provides the opportunity for skilled people to choose to be in one place their whole life."
McGowan said it would allow for greater clarity of infrastructure requirements.
"It allows better planning by knowing how many workers they have to cater for."
Investors have until March 29 to express interest in the Mt Pisa project.