The Brumbies have been hit by mumps ahead of this weekend's Super Rugby match with the Chiefs.
A number of cases have been confirmed, with Rugby Australia and Sanzaar stepping in to control the issue.
After consultation with ACT Health, only players and staff who are completely well will travel to Hamilton for Saturday's game.
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The initial testing conducted last week was inconclusive, with a second wave of testing confirming the presence of the virus among some players and staff.