Former All Blacks captain Graham Mourie and former All Blacks assistant coach Tony Gilbert will coach the teams in the first North and South match for 17 years.
The game will be held at Dunedin's Forsyth Barr Stadium on June 10 as a way to help the cash-strapped Otago Rugby Union, who recently staved off bankruptcy.
Mourie, who captained the North in six appearances between 1976 and 1982, will coach the North team with assistance from North Harbour coach Liam Barry.
Gilbert, the former Otago, Highlanders and All Blacks assistant, will take charge of the South with help from former All Black and current Otago coach Tony Brown.
Players will be drawn from the five Super Rugby teams and wider training groups but the quality of the sides is questionable. They will be without All Blacks, who play Ireland in the first test of the year in Auckland on June 9, and many of the Super Rugby franchises have said they are reluctant to release players for the match.