Scoring a try in the 28-16 win, Nightingale has since added a further 109 tries to his NRL career tally to sit equal-sixth for most tries in the history of the Dragons, Steelers and St George Illawarra.
"It's a very proud and solemn day for me to be announcing the end of the career," said Nightingale.
"I am extremely grateful for everything that the Dragons and the game has done for myself, and what I've been able to achieve.
"There are so many people to thank for what's been a special journey. From Renown United through to the junior reps to now, through the highs and lows and great successes I've had, I'm just so thankful for this incredible journey."
Nightingale has remarkably won every single accolade awarded at the Dragons' end-of-year presentation.
Winning the prestigious Dragons Medal for player of the year in 2011, Nightingale also won the Immortals Trophy (2011), the Geoff Selby Memorial Trophy (2010, 2016), the Community Involvement Award (2012) and the Red V Members Player of the Year (2011, 2013).
Nightingale also won the Mark Coyne Trophy for career development in 2014, which has been reflected in his establishment of the Elite Athlete Business School.
"There have been so many great seasons personally," he said.
"To be recognised for so many different reasons that are celebrated at the Dragons, they all still sit on my mantelpiece very nicely.
"The 2010 grand final win was the highlight for me and my family. There are so many fond memories from that day and the season as a whole. Although now is great time to reflect, there are still plenty of memories to be made this season yet.
"However I'm also look forward to more family time with my second child on the way. My wife Bianka has sacrificed a lot for my career. She's been selfless by supporting me throughout my career, while also building one of her own.
"It'll be good to rearrange my priorities now and put her career first as well as continue to build the Elite Athlete Business School and help fulfil its potential alongside my business partner and the EABS family."
Dragons CEO Peter Doust congratulated the one-club player on his stellar career.
"Jason has always represented himself, his family and the Dragons with distinction and has always been committed to supporting the club's visions both off and on the field," said Doust.
"He is an outstanding role model who typifies the club's values and we have been privileged that he has remained loyal to the Dragons for so long. He signed eight contracts to remain at the Dragons.
"We are a development club who have always invested and provided opportunities for players in the local area and he epitomises everything we stand for. We couldn't be prouder of what Jason has achieved throughout his career."
Dragons coach Paul McGregor paid tribute to Nightingale's contribution and commitment to the club.
"It's hard to beat a person who never gives up and Gyspy typifies that," said McGregor.
"He's very tough and very committed. If you look at his career, what he's done season after season is quite incredible.
"He's been fortunate enough along the way to avoid too many injuries and has represented his country with pride every time he's donned the Kiwis jumper.
"I've known Jason for a very long time so I feel very privileged to have had the honour as a head coach to watch this young man develop into a father, a husband and one of the best clubmen we've had over a long period of time."
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