Apart from Kahui, Rennie is also missing halfback Brendon Leonard (cheek bone) and fullback Robbie Robinson and midfielder Andrew Horrell (both foot injuries).
Charlie Ngatai, a midfield signing from the Hurricanes, left the field against the Reds a fortnight ago with a groin injury, but has recovered sufficiently to be named on the bench.
The Chiefs were rocked by injuries after their first round loss to the Highlanders in Hamilton last season. This time they have come a little earlier.
"We only lost Andrew this time last week," Rennie said. "He's out for probably another month. Robbie has had issues with his foot for a while now and it's been slow going, same with Richard's shoulder. Obviously when we selected our team a few months back they were going to be a big part of our starting group but we've got a lot of depth and flexibility in our group.
"We picked four midfielders and we wouldn't have thought we'd be starting with Tim but he did a great job for us against the Hurricanes last weekend and we think he can do the same again this week."
In another departure from the norm, former Blues first-five Gareth Anscombe has been named at fullback.
"We see him as a 10," Rennie said. "We've talked to him about the value of playing more than one position. If you look at someone like Beauden Barrett, he got an All Black opportunity based on the fact that he can play multiple positions. It's good for Gareth, he's quick enough to play on the wing, he's quick enough to play at the back ... he can expose teams from a broken field point of view."
Wing Patrick Osborne, a signing from Canterbury, will also make his Super rugby debut. Rhys Marshall, a hooker from Hawkes Bay who has yet to play in ITM Cup, is in line to make his from the reserves bench.
Chiefs v Highlanders
Forsyth Barr Stadium, Dunedin
Tomorrow 7.35pm
Chiefs: Gareth Anscombe, Patrick Osborne, Tim Nanai-Williams, Bundee Aki, Asaeli Tikoirotuma, Aaron Cruden, Tawera Kerr-Barlow, Fritz Lee, Sam Cane, Liam Messam, Brodie Retallick, Craig Clarke (c), Ben Afeaki, Hika Elliot, Pauliasi Manu. Reserves: Rhys Marshall, Ben Tameifuna, Michael Fitzgerald, Tanerau Latimer, Augustine Pulu, Charlie Ngatai, Lelia Masaga.
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