English trainer Ed Dunlop has described the death of Red Cadeaux as his saddest day in racing.
The three-time Melbourne Cup runner-up was euthanised when irreversible complications arose concerning blood flow to his left foreleg which he injured in this year's race.
Racing Victoria said vets at the University of Melbourne Clinic at Werribee advised the problem could not be successfully treated.
"This is my saddest day in racing. Red Cadeaux was a much loved member of our family and my thoughts are with his owner Mr Arculli and my staff, particularly Robin Trevor-Jones and Steve Nicholson, who spent every day caring for this horse," Dunlop said in a statement.
"Unfortunately the complication was irreversible and the decision to euthanise the horse, whilst terribly hard, was made in his best interests.