Jo Aleh and Polly Powrie have had their protest against disqualification from yesterday's race 6 of the women's 470 thrown out after they failed to lodge their appeal in time.
The decision scuppers their chances of defending their Olympic title given they have incurred two disqualifications but they are still an outside chance of a minor medal.
They were ruled to have crossed the startline early and followed a disqualification on the opening day of racing, when they were penalised for a port-starboard incident.
Both disqualifications carried the maximum 21 points, and saw the defending Olympic champions take a slide down the standings to seventh overall. Had the penalties not been imposed, the pair known as Team Jolly would have been in a share of the lead.
Aleh and Powrie appealed the jury's decision in the first race over the weekend after video evidence of the incident with an Austrian crew surfaced, but they could not convince the five-person panel to budge from their decision.