Despite walking back to the track's medical centre on his own, Hartley went to hospital for precautionary scans.
"I'm definitely fit, healthy, and ready for the next race," Hartley said in a press release.
"I'm really disappointed for Toro Rosso and Honda, because we've been quite strong all weekend with a good update from the power unitm" he added.
"I think we definitely had the pace to challenge for points today. I had a good start, similar to the drivers around me, but I got left a bit on the outside of Turn 2 which lost me a position. I got a good run on Lance out of Turn 4 on the outside, but he lost the car which put us both into the wall. It's very disappointing as there was room to make the overtake, and I was looking forward to a strong race."
Stroll was disappointed to miss out on completing his home event and explained the incident as he saw it.
"I had a good start and gained a few positions," the Canadian said. "I was up to 13th.
Going into turn five I was side by side with Brendon – the car got loose on me. I corrected it but it was too late and I touched him. There wasn't enough room for both of us so into the wall."
Stroll felt neither driver was really at fault.
"It is racing – things like that happen. First lap, you are close, side by side and that is how it goes some time."
It is a cruel blow to Hartley's Formula 1 hopes. He had been under serious pressure to keep his seat with rumours surfacing this week that his Toro Rosso team approached Formula 2 star Lando Norris to take over the drive.
Hartley's improved pace in Montreal came at a crucial time as he out-qualified teammate Pierre Gasly with a new engine package.
Ferrari's Sebastian Vettel led the entire race to take the lead of the championship standings.
"Perfect is a good way to describe it," Vettel said. "I said yesterday how much this place means for Ferrari. To have a race like we had today was unbelievable. After a long stretch that Ferrari didn't win here, I looked around and the people were happy.
"There's still a long way to go [in the championship] so I'm not too bothered. [Getting to the lead of the championship] is a good side-effect."
Mercedes driver Valtteri Bottas finished second with the Red Bull Renault of Max Verstappen completing the podium.
Daniel Ricciardo was fourth in the second Red Bull while reigning world champion Lewis Hamilton settled for fifth in his Mercedes.
Hartley's Toro Rosso teammate Pierre Gasly just missed out on a point-scoring finish in 11th.