"Congratulations, again, Lydia. Another major," Spieth said to the Kiwi.
"We were all tuned in last week from Houston. I was watching and we all knew it was going to happen."
Ko, who was mobbed by fans and players seeking autographs, said she was excited to meet the American star.
Lydia Ko tees off on the par three ninth hole at Augusta. Photo / Getty
"Jordan's such a great player and I've always wanted to meet him," Ko told the New Zealand Herald.
"This is my first trip to Augusta and it's just amazing. Everything is perfect, you can see why everyone loves the Masters."
Lydia Ko walks with Kevin Na during the par three competition. Photo / AP
The fun didn't stop there for Ko either. The relaxed nine-hole Par 3 event often sees players invite friends and family to take a putt on the green.
But PGA Tour winner Na, who was playing the Par 3 with New Zealand's Danny Lee, went a step further and invited his caddy Ko to take a swing at the ninth hole - a 123m shot completely over water.
True to form, Ko grabbed Na's 9-iron and stuck her shot two feet from the pin, spinning the ball back and pulling up agonisingly short.
"That would have been my first hole-in-one ever," Ko said. "Wouldn't have been a bad way to do it at Augusta either."
World number one Ko also got to meet PGA Tour stars Rickie Fowler and Justin Thomas.
Ko, who is in Augusta to collect an award from the Golf Writers Association of America, was invited by members to have her first look around the hallowed property today - and ended up caddying for American PGA Tour player Kevin Na in the Par 3 tournament, decked out in the iconic white boiler suit.
The Masters starts tomorrow morning (NZT), with world number 38 Lee paired with Scotland's Russell Knox and American Smylie Kaufman.