The Heat said the injury was a bruised left knee.
Wade has battled knee trouble for several years and had just had the same knee drained last Saturday.
"It could have been worse," said Wade, who said he still hoped he'd be ready to play at Detroit on Saturday.
"I knew I was in trouble on my way down."
Any time without Wade will hurt the Heat, who have dropped two straight games as they take part in a five-team battle for the final two places in the Eastern Conference playoffs.
Brooklyn, Miami and Boston are all on 34 wins, with the Nets holding a game in hand. Behind them are Charlotte and Indiana, who have 32 wins apiece.
In Oakland, the league-leading Golden State Warriors made it 11 wins in a row as they beat the Phoenix Suns 107-106 with a basket at the buzzer.
Trailing by one point with four seconds left, the Warriors inbounded the ball to a driving Harrison Barnes who made the basket with two-tenths of a second on the clock, triggering rapturous celebrations at Oracle Arena.
Steph Curry again led the scoring for the Warriors with 28 points as he shot six from 11 from beyond the arc, including a dagger three-pointer after Andrew Bogut collected a vital disputed rebound with eight seconds left on the clock to keep Golden State in the game.
In the day's other game and a possible preview of a first-round playoffs match-up, Houston moved into second in the West with a 108-101 victory over the Dallas Mavericks.
James Harden had 24 points in the win, while Detroit outcast Josh Smith continued his rapid improvement at the Rockets with 15 points and 12 rebounds.
- AAP
