More than 1000 St John Ambulance officers nationwide have issued a 14-day notice of industrial action.
The First Union says the planned strike comes after an attempt by St John to squeeze more out of already stretched front-line staff.
The union's ambulance professionals co-ordinator, Lynette Blacklaws, says the officers will continue to respond to call-outs.
Their industrial action would include a ban on paperwork, disrupting St John's ability to invoice patients.
Ms Blacklaws, says the strike is for the safety of staff and their patients.