Ms Almao said the men were in "much better shape" than when last she saw them, and were feeling "much stronger".
"I think because of the conditions under which they are here, with full protection and assistance from the judicial authorities, that they are feeling very strong about giving their testimony," she said.
"They want to give their testimony to bring Sir Peter's murderers to justice."
Ms Almao said the trial would resume with a reading of the charges -- first in Portuguese, then in English.
The judge would direct which witness he wanted to hear from first.
The three crewmembers and the Brazilian cook who was also aboard when Sir Peter died, would be called to testify.
Ms Almao said the three New Zealanders were expected to have finished giving evidence in time to return to New Zealand on Wednesday local time.
The trial, however, would continue.
The judge is expected to give his ruling in June or July, possibly earlier.
The court case began a fortnight after Sir Peter's murder, following a Brazilian government directive to local authorities to give the investigation top priority.
It was adjourned for the Christmas/New Year holiday recess.
Ms Almao said she was in Macapa to support the New Zealand crew and monitor the trial.
The murder of 53-year-old Sir Peter, renowned internationally for his yachting achievements, sent shockwaves around the world and propelled New Zealand into mourning.
About 30,000 people attended his memorial service in Auckland just before Christmas.
Seamaster was on the Amazon River as part of Sir Peter's latest venture, blakexpeditions, formed to help raise international awareness of threatened areas of the environment.
- NZPA
Peter Blake, 1948-2001