Two kiwifruit orchards in the Gisborne district have now been confirmed as having the Psa-V vine-killing disease.
Further test results have confirmed the outbreak on the first orchard where it was found at Te Karaka, inland from the city, while a second affected orchard was identified this week.
"Kiwifruit Vine Health has introduced a total exclusion zone for the whole of the Poverty Bay flats area now, as a result of the second outbreak," said Gisborne regional committee member Bill Thorpe.
That move requires growers to apply prescribed management systems and prohibits the movement of plant material to or from orchards.
"One of the transmitters for Psa-V is organic matter, which obviously includes plants and pollen."