"The Resource Management Act clearly states trade competitors have no place in the resource consent process.''
In addition to obtaining resource consent from the Queenstown Lakes District Council and the Otago Regional Council, the company also requires a fish farm licence.
Mr Lee said Fish and Game was opposing the licence application, causing "further costly delays''.
"These obstructive actions by Fish and Game are an obvious abuse of [the] statutory manager's powers and a misuse of public funds.
"Fish and Game are utilising public funds without accountability to stymie developments that are of benefit to the very same public supplying their funds.''
Mr Lee said he was complaining to Ms Barry because Fish and Game was given the title of "statutory managers of game fish'' under the Conservation Act.
"We believe to create a level playing field and remove this rort of the system, Fish and Game must be removed of the ... title.''
Otago Fish and Game chief executive Niall Watson yesterday denied there was any "trade competition element'' in the organisation's position on Mr Lee's fish-out proposal.
"In fact, a fish-out pond could have a beneficial effect on licence sales by introducing members of the public to angling as a recreational pursuit.''
Mr Watson said his organisation's main concern was with the effect of the development on water quality.
"Fish and Game still considers that downstream water quality is the key issue and that Mr Lee's proposed fish-out pond stocking rates and maintenance feeding regime will degrade water quality.
"That view has been supported by assessments by independent consultants commissioned by Fish and Game ... but he [Mr Lee] doesn't accept there is an issue even though it could affect the viability of the proposal.
"In my view, he would be well advised to seek a second opinion on the water quality implications of his proposed operation rather than berating Fish and Game or complaining to ministers of the Crown.''
Fish farming licences are issued by the Ministry for Primary Industries and Mr Watson said while Fish and Game had raised its concerns over water quality with the ministry, it had "not been formally consulted'' about the licence application.