"And there's no better example of a scorned woman than Amandeep Kaur," Ms Jayanandan said.
"A jilted woman, a scorned woman; scorned by her own family, her husband, even her work colleagues."
The defendants had an affair for several months while they worked together at Sistema Plastics in Penrose.
Three weeks before the alleged murder their respective spouses found out and the Crown says a plot to kill Davender Singh was hatched by the duo so they could be together.
The plan allegedly materialised when Gurjinder Singh followed the couple in their car as they left work and the attack occurred after they had pulled over on Norman Spencer Drive.
During the Crown closing yesterday, prosecutor Natalie Walker said the victim was a strong man and could not have been killed by his wife unaided.
But Ms Jayanandan disagreed.
"It's playing on your preconceived notions of women being the weaker sex," she said.
"Women can and will kill."
She accepted that outside the car Davender Singh would have had the upper hand but with the element of surprise in close confines it was not inconceivable that Kaur could have killed her husband alone.
Gurjinder Singh told the jury the fatal blow had been struck when he turned up to talk to Davender Singh and reassure him the affair was over.
He only took the knife and victim's cellphone from the scene because Kaur threatened to implicate him, he said.
Ms Jayanandan said Kaur's marriage was "miserable" and she saw her co-accused as a way out.
"She tried to keep him hooked with sexually-explicit text messages", and the pair's exchanges showed the imbalance in their relationship, she said.
Ms Jayanandan accepted her client's "alleged confessions" during police interviews did not look good but she said he was just trying to end the gruelling questioning.
She said the science spoke volumes and the blood found at the scene and on clothing all pointed to Kaur being the killer.
"She had the motive, she had the opportunity and she had the element of surprise."
Kaur's lawyer will close his case today before Justice Lang sums up the case.