The results were recorded into a specific software program and analysed by K9MD's biostatistician.
"Using various concentration ratios from 100 per cent down to 5 per cent for the validation, Frieda successfully detected positive prostate cancer samples 100 per cent of the time and ignored samples that did not contain cancer 100 per cent of the time," Blomfield said.
"Since validation, Frieda is now detecting positive prostate cancer samples as low as 1.25 per cent."
The validation announcement coincides with Prostate Cancer Awareness Month (November).
Blomfield believed dogs such as Frieda were "the way forward" for early detection, to offer New Zealand men an easy, non-invasive screening and surveillance opportunity for prostate cancer.
"This would be a value-added tool to help protect men's health, enable earlier instigation of treatment and potentially lead to improved patient outcomes.
"The use of highly trained medical detection canines will help decrease the need for any extra invasive tests and it will help increase the efficiency of the health system."
With Frieda's solid odour imprinting of the specific volatile organic compounds released from prostate cancer, K9MD planned to continue with the second stage of her training, using patient urine samples.
However, more fundraising was required first, she said.
About $250,000 per year was needed to continue the work.
Earlier this year, K9MD dog Levi achieved a world-first when he successfully completed the proof-of-concept stage of training by detecting bowel cancer in saline as low as 6 per cent ratio.
Levi's training has now moved to stage two - diagnostic test accuracy in real-world conditions using patient urine samples.