The court heard that two attempts to use the card were made at an ATM in Mt Albert and two more 30 minutes later at an ATM in St Heliers.
Fraud investigator Steven Bleakley said all four attempts were unsuccessful because the wrong pin number was used.
The court watched CCTV footage of a man trying to use Li's card at the eftpos machine in St Heliers, where he attempted to make a withdrawal of $1000.
Mr Bleakley said the card was retained after the fourth failed attempt. He said that was standard procedure.
He also told the court that Yu's bank account had a balance of $27.45 on May 17 2010.
During the Crown's opening on Monday, prosecutor Aaron Perkins said Yu thought he knew Kiko Li's pin number but he was wrong.
"The Crown case is that you will have little doubt that Yu is at the second ATM trying to get money."
The trial is set down for three weeks and will hear from 44 witnesses.
Yu's friend Yongxin Li pleaded guilty to murdering Kiko and has been sentenced to life in prison with a minimum non-parole period of 16 years.