"We're free to assemble because Antarctica remains Covid-19 virus free, so we have no restrictions on gatherings like back home," he said.
In his Anzac service address, O'Connor reflected on the spirit of World War I soldiers which has since become part of New Zealand's national identity.
"We use it now to work for peace and tolerance and co-operation," he said.
"The world is now in great need of co-operation. The Anzac traditions continue today at Scott Base which is home to both Kiwis and Aussies. We come together and appreciate ingenuity, good humour and mateship. We also hope to be strong in times of adversity."
Antarctica New Zealand chief executive Sarah Williamson said this year's service seemed even more special than usual.
"Antarctica New Zealand has a close partnership with the New Zealand Defence Force who support our operations on ice, so it is important to be able to remember the sacrifice made by members of our military all those years ago," she said.
"On a personal note, it is heartening to know that Kiwis and Australians – supported by our American friends - are gathering together to mark this occasion.
"The Scott Base crew are coming together in commemoration at the bottom of the world, on behalf of all of us back home."
Around 30 people from the nearby American base McMurdo Station also attended including a trumpeter who played The Last Post and reveille, which was followed by wreath laying. The Ode of Remembrance was read by winter engineering supervisor and electrician Luke Keehan.
Antarctica New Zealand says there are no personnel movements to and from Antarctica planned for a number of months, which is normal for the winter season.
But keeping Antarctica Covid-19 free and the safety of its staff remains its priority.
"We are assessing our approach for the end of the winter, when personnel movements usually resume. This is a number of months away. Any protocol and procedures put in place in the future will be to protect Antarctica, Scott Base and our staff."
• Covid19.govt.nz: The Government's official Covid-19 advisory website
RSA national president BJ Clark sent the Scott Base team a note before the unique service to acknowledge their efforts and to wish them well.
"It shows once again that Kiwis wherever they are in the world still take the time to remember," he said.
"Here's a group of people in a winter-over situation in the Antarctic, certainly isolated, yet they're still taking the time to plan and hold a service to remember those who have served and those who are still serving today."
