Safe now wants a blanket ban on events at council facilities that they believe promote animal cruelty.
Campaign manager Mandy Carter said: "People in Hamilton are really disgusted about it. It's a really cruel way to treat animals - we wouldn't do it to dogs and cats so why would we do it to cattle and horses.
"It's not right and the council should join other councils like Auckland and ban rodeos from the land."
Auckland Council banned rodeos in 2008 but 35 outdoor, one-day rodeos are held throughout the country each year including in Kihikihi, Huntly and Whangarei.
Ms Carter urged the council to think about Hamilton's reputation.
"If you want to be seen as a progressive, cosmopolitan smart city you shouldn't be allowing events like that to take place."
But Rodeo Cowboys Association board member Fred Doherty rejected claims animals were mistreated and said a strict code of conduct was followed.
"We are not answerable to Safe, we don't care what Safe say, we are answerable to the National Animal Welfare Advisory Committee.
"We've never been in trouble, our animal welfare record is impeccable, stock is vetted in and out of the event, injuries are absolute minimal, deaths are incredibly rare and so we are completely above worry as far as that is concerned."
Mr Doherty, who is also the production manager for the international rodeo in Hamilton, said last year's event was a huge success.
"You couldn't run an event that successful and not come back."
A provisional date for the next event has been set for November.
Councillors will consider the petition on Thursday. They have been advised to defer the decision until a reviews of the council's event venue facilities policy, which will include a new ethical component, next month.
In a report to the council, social development team leader Helen Paki said there could be financial losses if the rodeo did not run at Claudelands.
Council finance and monitoring committee chairman Dave Macpherson said he felt rodeos bordered on the ethical threshold.
"I suspect a lot of other Kiwis are like that - they are not sure how much they exploit animals."