They claimed that the differences proved it wasn't actually Melania in Alabama with her husband.
"The recasting of Melania is the worst I've seen since they recast Aunt Viv in Fresh Prince #fakemelania," one Twitter user wrote.
"I clicked on #FakeMelania against my better judgement and I have to say that it DOES NOT look like Melania," another tweeted.
The claims about Melania using a body double at public events first arose in October 2017 when she appeared beside her husband as he addressed the media about hurricane relief for Puerto Rico.
During the press conference, Trump pointed out the presence of his wife even though she was standing in clear view of the cameras.
"My wife, Melania, who happens to be right here," he said.
Will the real Melania please stand up?
Is it me or during his speech today a decoy “stood in” for Melania??...
Posted by Andrea Andi Wagner on Friday, 13 October 2017
Conspirators have based their theory of a Melania body double on the fact that Trump pointed out the First Lady was there to cover for the fact she was actually somewhere else.
READ MORE: Footage reignites Melania Trump body double claims among conspiracy theorists
The theory resurfaced again in August last year during a trip to Columbus, Ohio with the President.
The conspiracy theories are often renewed when Melania appears alongside her husband wearing sunglasses or whenever a grainy photo of her emerges.
In Alabama on Friday, Melania wore sunglasses as she paid her respects to the victims in the tornado-ravaged area.
The Trumps then went on a helicopter tour of the area, visited the homes of some victims and went to a disaster relief center at the Providence Baptist Church in Opelika to meet with survivors, volunteers and first responders.