The bells of Westminster Abbey will not ring for the Duke, who missed out on the celebration last year because of Covid-19 restrictions but was given the honour in 2020, even after his Newsnight interview about his friendship with Jeffrey Epstein.
The decision, made temporarily in 2020, is "now permanent", a source said.
'No longer a requirement'
A number of authorities had already declined to honour the Duke's birthday, after his reported £12 million (NZ$24m) settlement of a sex abuse case brought against him proved the final straw in public opinion.
A spokeswoman for Westminster Abbey said: "Due to the financial challenges posed by the Covid-19 pandemic, Westminster Abbey will ring its bells only for the birthdays of HM The Queen and HRH The Prince of Wales."
In 2020, after emails insisting that local authorities fly the flag for the Duke's birthday were leaked, DCMS clarified that it was "no longer a requirement" for them to do so.
But the date, February 19, remained on the list of official "designated days" on its website until the end of 2021. On February 11, it discreetly removed the birthday for the 2022 calendar.
A government spokeswoman said: "Since 2021 the default position in Great Britain is that the Union Flag flies all year round unless another flag is being flown.
"However we routinely review the list of designated days for flying the Union Flag on UK Government Buildings.
"These changes make the days consistent with other commemorative events, such as gun salutes."
A source said the Duke would be spending Saturday "quietly at home".
His mother the Queen is in residence at nearby Windsor Castle and was this week receiving official guests after a Covid scare.
The Duchess of York lives at Royal Lodge and is expected to celebrate her ex-husband's birthday with him, with visits or video calls from his two daughters and grandchildren.