Luckily, this time things were different, though they felt spooked enough by the miscarriage to become obsessed with every little detail in the early days. Trystan began "obsessively" weighing himself and took multiple other pregnancy tests in the weeks to come.
When it came to the six-week ultrasound appointment, the couple called ahead to the ob-gyn's office to let them know in advance, and make a note in his chart, about how they would be treating a pregnant trans-dad.
Trystan felt it was a necessary precaution, based on his experience as a transgender individual.
"I can feel someone looking at my face and searching for the remnants of womanhood," he said.
"They kind of squint their eyes a little bit and I can tell they're trying to take away my beard, they're trying to de-transition me in their heads."
However, his fears were happily unfounded.
"There just hasn't been an ounce of transphobia from anyone I have come in contact with," he said. "Every person wouldn't even bat an eye that there was a dude with a beard claiming to be pregnant who is here to get bloodwork done - and I have been trans long enough to know that doesn't come magically."
Biff and Trystan first gained attention for sharing their journey to "accidental" parenthood after, in 2011, their niece and nephew were put into their care after Biff's sister and her boyfriend were deemed unfit to care for them due to substance abuse issues.
Riley and Hailey had plenty of emotional baggage from their previous neglectful environment, but thanks to Biff and Trystan are happy and healthy and loved.
As for the new addition to the family, they revealed that they will be soon welcoming a son, who they joke about in the family as being "diagnosis: male."