In a brief hearing at Central Local Court, Ms Shao did not apply for bail and bail was formally refused.
Ms Shao, who is being held in a Sydney women's prison, will appear again on October 31 when a Mandarin interpreter is required.
Her lawyer refused to comment outside the court on Ms Shao's condition in custody.
NSW Police say Ms Shao allegedly administered the cosmetic surgery last Wednesday on Jean Huang.
The procedure was allegedly undertaken at the Medi Beauty Clinic on in the inner city Sydney suburb of Chippendale owned by Ms Huang.
Ms Huang, who is believed to have suffered brain damage, died last Friday.
A NSW court heard last week that Ms Shao was on a tourist visa and not qualified to carry out cosmetic work in Australia at the time Ms Huang was injected.
The 33-year-old had only been in the country five days by the time she was taken into custody on Wednesday night following Ms Huang's collapse in the salon.
Ms Shao's arrest followed the procedure she allegedly performed at the Medi Beauty Clinic in the inner-city suburb of Chippendale. The procedure was to put fillers into each breast.
Police allege Ms Shao administered lidocaine, an anaesthetic, as well as an "intoxicating substance", the drug tramadol, into Ms Huang's chest.
It will be further alleged Ms Shao injected Ms Huang through a catheter and then injected the lidocaine into Ms Huang's chest, before injecting the "breast fillers".
When Ms Huang went into cardiac arrest she and two other staff members tried CPR until paramedics were able to revive her.
It's understood Ms Shao met Ms Huang through mutual friends and Ms Huang agreed she could perform the procedure.
Taken to Royal Prince Alfred Hospital last Wednesday, Ms Huang was revived but was not predicted to survive and died two days later.
Last week at Central Local Court, bail was opposed by prosecutors because Ms Shao posed an unacceptable risk to the community and was a flight risk as she had no ties to Australia and was facing serious charges.
Magistrate Sharon Freund said she believed the prosecution case was a strong one and if convicted Ms Shao faced a lengthy jail term.
The magistrate told the court Ms Shao had admitted to administering local anaesthetic and breast fillers to Ms Huang.
She denied Ms Shao bail, saying there was an unacceptable risk she would interfere with witnesses or "endanger" the community.
"In my view it's a very strong prosecution case and in the event that she's convicted ... I'm of the view that she will receive a custodial sentence," Ms Freund said.