A follow-up study involved analysing videos of high-profile press conferences in which people appealed for help finding relatives, or said they had been victims of crime.
Volunteers could not tell who was telling the truth and who was lying from their eye movements.
The study's co-author Dr Caroline Watt, of the University of Edinburgh, said: "A large percentage of the public believes certain eye movements are a sign of lying and this idea is even taught in organisational training courses.
"Our research provides no support for the idea and so suggests that it is time to abandon this approach to detecting deceit."