"It's an unlawful condition of entry, therefore that exposes you, personally and Bunnings to being sued for discrimination because it is in breach of the 1948 Charter of Human Rights to discriminate against men and women."
Despite being asked to stop filming several times, she refuses.
The video, which goes for three-and-a-half minutes, ends when a second woman joins the one filming, having picked up an item in store.
When asked if that is all, the woman filming retorts with: "No, no, no, we're going to have a wander".
It comes as Victoria recorded one of its highest coronavirus cases on Sunday, with 459 people testing positive and 10 deaths.
Commenting on the video on Facebook, Jessica Weiks described her as a "f***ing Karen".
"It's not your right Bunnings is not a public place they have their own conditions of entry exactly the same as when you to into a Big W store and they have a sign saying your bags will be checked as a condition of entry, it's not law it's their company's condition of entry," she wrote.
Bunnings chief operating officer Deb Poole described the woman's behaviour as "completely unacceptable" and said the safety of customers and staff was the chain's highest priority.
"We won't tolerate abuse against our team members and we have security at all metro Melbourne and Mitchell Shire stores as support.
"Our team will also alert the police should they encounter any difficulties."
Oh man, I just saw 10 seconds of Karen at Bunnings and had to turn it off. I honestly can't stand these anti maskers. It's not hard, nor particularly uncomfortable to wear one. Stop being a selfish self entitled twat. It's not forever#MaskUpMelbourne