Her agent said Chambers, who died from natural causes on Wednesday evening, would be 'greatly missed'.
The Vicar of Dibley actress Emma Chambers has died of natural causes aged 53, her agent has confirmed. She was best known for playing Alice Tinker in the BBC sitcom which starred Dawn French (right), the Doncaster-born star also featured in Notting Hill alongside Julia Roberts.
Jon Plowman, executive producer of The Vicar of Dibley and former head of comedy at the BBC, said: 'This is a sad day. Emma was a gifted comic actress who made any part she played - no matter how ditzy or other worldly - look easy.
'To create a much loved comic character as she did, you have to be every bit as bright and clever as Emma always was.
'She was great fun to work with and adored by all the cast and crew of Vicar of Dibley. She will be missed and our deepest condolences go out to her family and friends.'
Chambers played alongside French from 1994 to 2007 in the much loved sitcom and won the British Comedy Award for Best Actress for her performance in 1998.
Once asked if she resembled her portrayal of the dippy Alice Tinker in real life, Chambers rejected the comparison with the words: 'I'm a cynical old bitch.'
In Notting Hill, Chambers played Honey, Hugh Grant's eccentric younger sister and the romantic interest of Rhys Ifans' character Spike.
Her friend and fellow broadcaster Emma Freud, who is married to director Richard Curtis tweeted: 'Our beautiful friend Emma Chambers has died at the age of 53. We're very very sad.
'She was a great, great comedy performer, and a truly fine actress. And a tender, sweet, funny, unusual, loving human being.'
Vicar of Dibley co-writer Paul Mayhew-Archer, 65, said he was 'devastated' by her passing, which was said by her agent to be of natural causes.
'I loved working with her, she was stunning,' he said. 'I used to love watching her going over her lines in rehearsal, she would read them to herself and try to find the perfect delivery.
'I am devastated, she was a key part of the Vicar of Dibley. It is one of those strange things, because when you start working on something, you think it is one thing and it becomes something quite different.
'Alice became completely central to the piece, it was just perfect.
'Most sitcoms have an idiot of some sort but she managed to make her idiot completely different, it was amazing.
'The last time I saw her was the last episode, all of my memories are to do with the programme, her passing was so sudden.'
James Dreyfus, who starred alongside Chambers in Notting Hill paid tribute on Twitter.
He said: 'RIP the wonderful and talented Emma Chambers. Unique & unspeakably funny. Too young. Thoughts with her family.'
A statement from her agency read: 'Emma created a wealth of characters and an immense body of work. She brought laughter and joy to many.' She is pictured right with Dawn French on the Vicar of Dibley and right in How Do You Want Me?
Chambers, who lived in Lymington, Hampshire, is survived by her husband, fellow actor Ian Dunn, an actor in the daytime show Doctors.
She tied the knot in 1991 at a low-key ceremony in New Forest and described her marriage as 'glorious' in an interview in January this year.
'My wedding was very short. I was playing the lead in an Alan Ayckbourn play and was only given one day off,' she told Hitberry magazine.
'My sister, Sarah Doukas, is a model agent and one of her bookers made me a dress which cost £180. I still look at it in my wardrobe and think it is one of the most beautiful things I have ever seen.
'The wedding itself was in the New Forest. I loved it because I had never had a big party before as we had always been on the move when I was a child.'
Former Top Gear presenter Jeremy Clarkson recalled Chambers being 'very funny'.
He wrote on Twitter: 'I'm sad about Emma Chambers. Knew her when she was a kid in Doncaster. She was very funny.'
