An insider told TVLine that despite her making out like it was her decision to leave the series, Rose's departure was a "break-up" between the 34-year-old actress and producers.
"It wasn't 100 percent her decision," the source told the website.
"It was a break-up. She wasn't happy working on the show, and did that make her fun to work with? No.
"So everyone decided it would be in the best interests of the show, and for all concerned, if they parted ways.
"It just wasn't a good fit."
The source added Rose, who has previously starred in Orange Is The New Black, John Wick: Chapter 2 and Pitch Perfect 3, felt pressure due to the long hours and didn't enjoy living in Vancouver, where the show is based.
Rose announced in a press statement yesterday she would not reprise her role for the second season.
"I have made the very difficult decision to not return to Batwoman next season," Rose said in a statement.
"This was not a decision I made lightly as I have the utmost respect for the cast, crew and everyone involved with the show in both Vancouver and in Los Angeles.
"Thank you to everyone who made season one a success – I am truly grateful," she said.
There was severe backlash when Rose's casting was announced two years ago, which forced the actress to turn her comments off on Instagram for a period of time.
The hashtag #RecastBatwoman began trending on Twitter shortly after the announcement, with the campaign based on the idea that her sexuality was not technically "gay" because she self-identifies as gender fluid.
"Where on Earth did 'Ruby is not a lesbian therefore she can't be Batwoman' come from — has to be the funniest most ridiculous thing I've ever read," she posted on Instagram at the time.
"I came out at 12? And have for the past five years had to deal with 'she's too gay' – how do y'all flip it like that?"
She finished saying the controversy had been a "rollercoaster" she needed a break from social media, adding: "If you need me, I'll be on my Bat Phone."
Last September, Rose revealed she had undergone emergency surgery after herniating two discs performing stunts on the series, leaving her in "chronic pain". She said she almost severed her spinal cord.
"The studio and network are firmly committed to Batwoman's second season and long-term future, and we, along with the show's talented creative team, look forward to sharing its new direction, including the casting of a new lead actress and member of the LGBTQ community, in the coming months."