The Avengers star was joined by her co-star in the Marvel's movie, Jeremy Renner, when she acknowledged the prestigious accolade.
Jeremy - who plays Hawkeye in the motion picture - joked about having a horrible work experience with her.
He said: "Working with her was tremendously awful. For six months, she continuously threw me around, beat me, stepped on me and wiped the floor with my dignity and self-respect every day - all with a smile on her face.
"Now, finally. I get to step on you, sweetheart."
The 27-year-old actress has been gracing the big screen since she was 10 years old.
The New York-bred actress thanked her mother and grandmother, who were present, for "inspiring me to be a strong, modern, independent woman."