Seacrest, 43, has repeatedly denied the allegations and an investigation into the matter - which was conducted by an outside counsel hired by NBCUniversal - has already found there to be "insufficient evidence" to support the claims.
In a new statement, Seacrest denied the claims and questioned why the claims were published without talking to him.
"Much to my dismay, Variety didn't speak with me or bother to speak with other credible witnesses or even ask for any of the evidence that was obtained during the investigation when offered, all of which clearly challenged the veracity of the claims made against me."
"This person who has accused me of horrible things tried to buy her silence by asking for money on multiple occasions — I refused. I have worked extremely hard to achieve my success and I don't take my opportunities for granted.
"I don't want to accuse anyone of not telling the truth but in this case, I have no choice but to again deny the claims against me, remind people that I was recused of any wrongdoing, and put the matter to rest."
Hardy - who was interviewed three times by the independent investigator - admitted to feeling exasperated by the outcome of the investigation.
She told Variety: "I felt like by the third interview, it was obvious the investigator was whitewashing it for Seacrest's side."
But an E! spokesperson has defended the process, describing it as "comprehensive and thorough".
The spokesperson continued: "Over the course of a two month process, our outside counsel interviewed more than two dozen people regarding the allegations, including multiple separate meetings with the claimant.
"The investigator is an attorney with nearly 20 years experience and is highly regarded professionally. Any claims that question the legitimacy of this investigation are completely baseless."
Meanwhile, Seacrest 's attorney, Andrew Baum, added: "On January 31st the network notified us that their independent third-party investigation had concluded that there was insufficient evidence to support her claims, effectively, clearing my client's name.
"It's telling that after my client refused to pay her money, and the E! investigation resulted as it did, that she is now coming forward to share her debunked story to the press."
- Bang! Showbiz