Comedienne Kate Walsh joined in, saying: "Charlie is an amazing medical specimen. I guess that's what comes from waking up at the crack of crack."
Jon Lovitz later quipped: "How much blow can Charlie Sheen do? Enough to kill two and a half men."
Amy Schumer also made fun of the star by referencing his replacement on the TV show, Ashton Kutcher - who is married to Bruce Willis' ex-wife, Demi Moore.
She said: "Just like Bruce Willis, you were big in the 80s and now your old slot is being filled by Ashton Kutcher."
At the end of the evening, held at Sony Studios in Culver City, California, Charlie - who had taken everything in good humour throughout the night - remained unrepentant of his lifestyle choices.
He said: "Wow, what a night. Until tonight, I never realised how f***ed up I was. All this time I thought I was just having fun. The thing is once again, I come out unscathed. You can't hurt me. Hell, I can't even hurt me. Drugs couldn't kill me. Sex couldn't kill me. The press couldn't kill me. Two and a Half Men couldn't kill me. Did you really think your little jokes were going to kill me? I'm done with the winning cause I've already won. This roast may be over, but I'm Charlie Sheen.
"In here burns an internal fire. I just have to remember to keep it away from a crack pipe."
- BANG! Showbiz