The tweet read: "Yes they are sometimes necessary and lifesaving, but they should be a last resort. Pure laziness for those who would rather medicate than put in the time and effort to heal people's minds."
Attempting to clarify her position, Rowling then used 11 tweets to discuss mental health and the transgender community, stating her concerns surrounding hormone therapy.
She continued: "As I've said many times, transition may be the answer for some. For others, it won't – witness the accounts of detransitioners."
Rowling then posted a link to an account of a woman who has "detransitioned" after briefly living as a trans man.
Responding to the tweets, transgender model and activist Munroe Bergdorf slammed Rowling as "dangerous" and a "threat to LGBT people".
"Mark my words. JK Rowling is dangerous and poses threat to LGBT people," Bergdorf wrote.
"Trans healthcare is not conversion therapy. This is INSANE."
In a separate tweet she added: "JK Rowling is not a scientist. She is not a doctor. She is not an expert on gender. She is not a supporter of our community.
"She is a billionaire, cisgender, heterosexual, white woman who has decided that she knows what is best for us and our bodies. This is not her fight."
Others slammed the Harry Potter icon for her views:
Last month, JK Rowling faced widespread backlash after similarly posting a series of tweets about sex and gender.
The criticism started after the Harry Potter author called out the use of the phrase "people who menstruate" by sharing an op-ed article with that wording on Twitter.
After receiving fierce backlash for the tweet, Rowling doubled down on her stance and pushed back against accusations of transphobia.
She claimed she has been "empathetic to trans people for decades" and called accusations that she hates transgender people "nonsense".
Penning a lengthy essay on her website, Rowling defended her right to speak about trans issues, explaining the complex reasons for her interest in the subject and revealing painful details about her past abuse.
Her controversial comments on gender identity triggered a number of Harry Potter actors to speak out against her.